Source Themes

Fluorescence anisotropy as a means to determine extracellular polysaccharide hydrolase activity in environmental samples

Current approaches to measure the activities of microbial extracellular enzymes in aquatic environments are hampered by slow throughput or by differences between the structure of simple substrate proxies and macromolecules. Here we show that …

Electron paramagnetic resonance spectroscopy as a novel approach to measure macromolecule–surface interactions and activities of extracellular enzymes

The dynamics of high molecular weight organic matter in marine systems are influenced by molecular conformation, interactions with surfaces and susceptibility to enzymatic hydrolysis, parameters that are difficult to observe experimentally. Here we …

Dynamics of dissolved carbohydrates in the Chesapeake Bay: Insights from enzyme activities, concentrations, and microbial metabolism

The interactions between heterotrophic microbes and high-molecular-weight (HMW) dissolved organic carbon in estuaries are complex and poorly understood. This study examined the co upling between hydrolysis of HMW carbohydrates (polysaccharides) and …