Potential for microbial denitrification coupled with methanol oxidation found in abundant MAGs in Antarctic Peninsula sediments

Abstract

Denitrification accounts for a substantial nitrogen loss from environmental systems, shifting microbial composition and impacting other biogeochemical cycles. In Antarctica, rising temperatures cause increased organic matter deposition in marine sediments, which can significantly alter microbially mediated denitrification. To examine the genetic potential of microorganisms driving N-cycling in these sediments, benthic sediment cores were collected at two sites in the Weddell Sea, Antar ctica. DN A was extr acted fr om m ultiple depths at each site, resulting in the reconstruction of high-quality metagenome-assembled genomes (MAGs). F orty-se ven of these MAGs contained reductases involved in denitrification. MAGs belonging to the genus Meth yloceanibacter w ere the most abundant MAGs at both sites and all de pths, exce pt de pth 3–6 cmbsf at one site, wher e they wer e not identified. The a bundance of these Methyloceanibacter MAGs suggests the potential for nitrate-dri v en methanol oxidation at both sites. MAGs belonging to Beggiatoaceae and Sedimenticolaceae were found to have the genetic potential to produce intermediates in denitrification and the complete pathway for dissimilatory nitrate reduction to ammonia. MAGs within Acidimicrobiia and Dadabacteria had the potential to complete the final denitrification step. Based on MAGs, Antarctic peninsula sediment communities have the potential for complete denitrification and dissimilatory nitrate reduction to ammonia via a consortium. Ke yw ords: Acidimicrobiia; Beggiatoaceae ; metagenomics; Methyloceanibacter ; nitrogen cycling; Sedimenticolaceae